gaussian filter dog fiji imagej plugin n a imaris v9 6 bitplane rrid scr 007370 graphpad prism v10 graphpad rrid scr 002798 other anoxic chamber (Oxford Instruments)
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Gaussian Filter Dog Fiji Imagej Plugin N A Imaris V9 6 Bitplane Rrid Scr 007370 Graphpad Prism V10 Graphpad Rrid Scr 002798 Other Anoxic Chamber, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SYBR Green Assay:Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: .. S100β rabbit Sigma-Aldrich S2644 Alexa Fluor secondary antibodies (donkey anti-species 488, 546, 633- conjugated) Invitrogen Biotium secondary antibodies (donkey anti-species 488, 546, 633-conjugated) Biotium Chemicals and other reagents Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante Equipment Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Microscopy:Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: .. S100β rabbit Sigma-Aldrich S2644 Alexa Fluor secondary antibodies (donkey anti-species 488, 546, 633- conjugated) Invitrogen Biotium secondary antibodies (donkey anti-species 488, 546, 633-conjugated) Biotium Chemicals and other reagents Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante Equipment Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante .. Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Spectrophotometry:Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: .. S100β rabbit Sigma-Aldrich S2644 Alexa Fluor secondary antibodies (donkey anti-species 488, 546, 633- conjugated) Invitrogen Biotium secondary antibodies (donkey anti-species 488, 546, 633-conjugated) Biotium Chemicals and other reagents Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante Equipment Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante .. Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Real-time Polymerase Chain Reaction:Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: .. S100β rabbit Sigma-Aldrich S2644 Alexa Fluor secondary antibodies (donkey anti-species 488, 546, 633- conjugated) Invitrogen Biotium secondary antibodies (donkey anti-species 488, 546, 633-conjugated) Biotium Chemicals and other reagents Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante Equipment Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante .. Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Software:Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: .. S100β rabbit Sigma-Aldrich S2644 Alexa Fluor secondary antibodies (donkey anti-species 488, 546, 633- conjugated) Invitrogen Biotium secondary antibodies (donkey anti-species 488, 546, 633-conjugated) Biotium Chemicals and other reagents Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante Equipment Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Article Title: Physiological and injury-induced microglial dynamics across the lifespan. Article Snippet: The institutions have accreditation from AAALAC and all experiments were performed within its guidelines. .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Paraformaldehyde VWR 101176–014 Sucrose Sigma S7903 DAPI Fluoromount-G Southern Biotechnology 0100–20 Phosphate buffered saline Sigma P3813 Isoflurane (Fluriso) Patterson Veterinary 07-885-2930 Dental Cement (C&B-Metabond®) Patterson Dental 553–3484 Betadine Fisher Scientific NC1696484 Buprenorphine Patterson Veterinary 07-894-9214 Cyanoacrylate glue (Loctite 401) Uline S-15623 Ophthalmic ointment Amazon B0CGY3XXFT NaCl Sigma S9625 KCl Sigma 793590 Glucose Sigma D9434 HEPES Sigma H3375 CaCl Sigma 1023780500 MgCl2 Sigma M8266 Fina Biosolutions LLC AMINO-DEXTRAN 2000 KDA X150, 100MG Fisher Scientific NC1275021 Alexa Fluor 680 carboxylic acid, succinimidyl ester Thermofisher A20008 4% PFA Thermo Fisher J19943.K2 Sucrose Milipore-sigma S7903-1KG OCT compound Fisher Healthcare 23-730-571 Sodium azide Sigma S2002 Experimental models: Organisms/strains CX3CR1-GFP/+ mice Jax ID: 005582 B6.129P2(Cg)-Cx3cr1tm1Litt/J Software and algorithms ImageJ, Fiji Version 2.1.0/1.53c https://imagej.net/ij/ N/A ImageJ Plugin for Article Title: Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium. Article Snippet: Article Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis. Article Snippet: Reagent/resource Reference or source Identifier or catalog number Goat anti-PIP5Kα Santa Cruz Biotechnology Cat # sc-11775 Goat anti-SUR1 Santa Cruz Biotechnology Cat # sc-11226 Mouse anti-PIP2 Echelon Cat # Z-A045 Rabbit anti-GFP MBL Cat # 598 Mouse anti-LC3 Cosmo Bio Cat # CTB-LC3-2-IC Rabbit anti-BKCa Alomone Labs Cat # APC-151 Rabbit anti-CaV2.3 Alomone Labs Cat # ACC-006 Mouse anti-syntaxin-1 Millipore Cat # MAB336 Mouse anti-Na/K ATPase beta 2 BD Transduction Labs Cat # 610914 Mouse anti-paxillin BD Transduction Labs Cat # 610051 Mouse anti-Hsc70/Hsp70 Enzo Cat # ADI-SPA-822-D Rat anti-Hsp90 Enzo Cat # ADI-SPA-835-D Rabbit anti-STIP1 Proteintech Cat # 15218-1-AP Rabbit anti-calnexin-1 Proteintech Cat # 10427-2-AP Mouse anti-derlin-1 Sigma Aldrich Cat # SAB4200148 Mouse anti-α-tubulin Sigma Aldrich Cat # CP06 Mouse anti-insulin Sigma Aldrich Cat # I-2018 Rabbit anti-phospho Src Epitomics/ Abcam Cat # ab32078 Rabbit anti-KIF5B This laboratory (Tanaka et al, 1998) Horseradish-peroxidase-conjugated protein A Cytiva Cat # NA9120V Horseradish-peroxidase-conjugated sheep anti-mouse IgG Cytiva Cat # NA931V Alkaline-phosphatase-conjugated goat anti-rabbit IgG, goat antimouse IgG, goat anti-rat IgG Cappel Cat # 59298, 59296, 59291 Alkaline-phosphatase-conjugated rabbit anti-goat IgG Zymed Cat # 61-1622 Alexa-conjugated goat and donkey anti-rabbit IgG, goat and donkey anti-mouse IgG, donkey anti-goat IgG Thermo Fisher Oligonucleotides and other sequence-based reagents PCR primers This study Methods Chemicals, enzymes, and other reagents Bay-K 8644 Selleck Cat # S7924 Alexa-conjugated phalloidin Thermo Fisher Cytochalasin B Sigma Aldrich Cat # C6762 Ionomycin Sigma Aldrich Cat # 19657 Nifedipine Sigma Aldrich Cat # N7634 SNX482 Alomone Labs Cat # RTS-500 L-ascorbate Sigma Aldrich Cat # A4034 Cycloheximide Fuji-Film WAKO Cat # 037-20991 Leupeptin Fuji-Film WAKO Cat # 336-40413 MG-132 Enzo Cat # P1102 CanGetSignal Immunostain Solution A TOYOBO Cat # NKB-501 CanGetSignal Immunoreaction enhancer Solution 1 and 2 TOYOBO Cat # NKB-101 Paraformaldehyde Fuji-Film WAKO Cat # 162-16065 Reagent/resource Reference or source Identifier or catalog number Glutaraldehyde Nakalai Tesque Cat # 170-03 Triton X-100 ICN Cat # 2923E Brefeldin A Sigma Aldrich Cat # B6542 Fluo4-AM Dojindo Cat # F311 Protein A Sepharose beads fast flow Cytiva Cat # 17-5132-01 μMACS Protein A microbeads Miltenyi Biotech Cat # 120-000-396 SYBR Primix Ex Taq (Tli RNaseH Plus) TaKaRa Cat # RR420 Software ZEN ZEISS ImageJ ver. .. 1.54i https://imagej.net/ Article Title: Ectopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion Article Snippet: Cytosine Arabinoside (AraC) Sigma-Aldrich C4645 Everbrite TM Hardset mounting medium with DAPI Biotium 23004 Isoflurane Abbott Laboratories Methanol 20% Mowiol NaN3 Normal Donkey Serum Paraformaldehyde (PFA) 4% Sigma-Aldrich P6148 PBS 1x Triton X-100 SYBR Green Thermo 4472918 Forward/Reverse Primers Lab supplies NucleoSpin® RNA/Protein kit MACHEREY-NAGEL Taq Polymerase EPO702 ThermoScientific LSG Dithiothreitol (DTT) Random Primers 5x Reaction Buffer dNTPs Isopentante .. Confocal microscope Leica Sp8 Confocal microscope Leica Sp5 Cryostat Leica CM1900 Stereotaxic apparatus Stoelting Silk Stitches Silcam/ Medipak Scissors FST Superfrost PLUS microscope slides Thermo Scientific Forceps FST NanoDrop1000 spectrophotometer Thermo Step-One Plus Real-Time PCR System Applied Biosystems MicroCL 21 Centrifuge Thermo Step Oneplus Real-Time PCR system Thermo Fisher Scientific applied biosystems Leica Ultracut R ultramicrotome Leica Microsystems, Wetzlar, Germany Diatome diamond knife Diatome Olympus Morada digital camera Olympus Software and Article Title: Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling. Article Snippet: Article Basal spot junctions of Drosophila epithelial tissues respond tomorphogenetic forces and regulate Hippo signaling Recombinant:Article Title: Physiological and injury-induced microglial dynamics across the lifespan. Article Snippet: The institutions have accreditation from AAALAC and all experiments were performed within its guidelines. .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Paraformaldehyde VWR 101176–014 Sucrose Sigma S7903 DAPI Fluoromount-G Southern Biotechnology 0100–20 Phosphate buffered saline Sigma P3813 Isoflurane (Fluriso) Patterson Veterinary 07-885-2930 Dental Cement (C&B-Metabond®) Patterson Dental 553–3484 Betadine Fisher Scientific NC1696484 Buprenorphine Patterson Veterinary 07-894-9214 Cyanoacrylate glue (Loctite 401) Uline S-15623 Ophthalmic ointment Amazon B0CGY3XXFT NaCl Sigma S9625 KCl Sigma 793590 Glucose Sigma D9434 HEPES Sigma H3375 CaCl Sigma 1023780500 MgCl2 Sigma M8266 Fina Biosolutions LLC AMINO-DEXTRAN 2000 KDA X150, 100MG Fisher Scientific NC1275021 Alexa Fluor 680 carboxylic acid, succinimidyl ester Thermofisher A20008 4% PFA Thermo Fisher J19943.K2 Sucrose Milipore-sigma S7903-1KG OCT compound Fisher Healthcare 23-730-571 Sodium azide Sigma S2002 Experimental models: Organisms/strains CX3CR1-GFP/+ mice Jax ID: 005582 B6.129P2(Cg)-Cx3cr1tm1Litt/J Software and algorithms ImageJ, Fiji Version 2.1.0/1.53c https://imagej.net/ij/ N/A ImageJ Plugin for Article Title: Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium. Article Snippet: Article Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium Article Title: Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling. Article Snippet: Article Basal spot junctions of Drosophila epithelial tissues respond tomorphogenetic forces and regulate Hippo signaling Saline:Article Title: Physiological and injury-induced microglial dynamics across the lifespan. Article Snippet: The institutions have accreditation from AAALAC and all experiments were performed within its guidelines. .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Paraformaldehyde VWR 101176–014 Sucrose Sigma S7903 DAPI Fluoromount-G Southern Biotechnology 0100–20 Phosphate buffered saline Sigma P3813 Isoflurane (Fluriso) Patterson Veterinary 07-885-2930 Dental Cement (C&B-Metabond®) Patterson Dental 553–3484 Betadine Fisher Scientific NC1696484 Buprenorphine Patterson Veterinary 07-894-9214 Cyanoacrylate glue (Loctite 401) Uline S-15623 Ophthalmic ointment Amazon B0CGY3XXFT NaCl Sigma S9625 KCl Sigma 793590 Glucose Sigma D9434 HEPES Sigma H3375 CaCl Sigma 1023780500 MgCl2 Sigma M8266 Fina Biosolutions LLC AMINO-DEXTRAN 2000 KDA X150, 100MG Fisher Scientific NC1275021 Alexa Fluor 680 carboxylic acid, succinimidyl ester Thermofisher A20008 4% PFA Thermo Fisher J19943.K2 Sucrose Milipore-sigma S7903-1KG OCT compound Fisher Healthcare 23-730-571 Sodium azide Sigma S2002 Experimental models: Organisms/strains CX3CR1-GFP/+ mice Jax ID: 005582 B6.129P2(Cg)-Cx3cr1tm1Litt/J Software and algorithms ImageJ, Fiji Version 2.1.0/1.53c https://imagej.net/ij/ N/A ImageJ Plugin for Ointment:Article Title: Physiological and injury-induced microglial dynamics across the lifespan. Article Snippet: The institutions have accreditation from AAALAC and all experiments were performed within its guidelines. .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Paraformaldehyde VWR 101176–014 Sucrose Sigma S7903 DAPI Fluoromount-G Southern Biotechnology 0100–20 Phosphate buffered saline Sigma P3813 Isoflurane (Fluriso) Patterson Veterinary 07-885-2930 Dental Cement (C&B-Metabond®) Patterson Dental 553–3484 Betadine Fisher Scientific NC1696484 Buprenorphine Patterson Veterinary 07-894-9214 Cyanoacrylate glue (Loctite 401) Uline S-15623 Ophthalmic ointment Amazon B0CGY3XXFT NaCl Sigma S9625 KCl Sigma 793590 Glucose Sigma D9434 HEPES Sigma H3375 CaCl Sigma 1023780500 MgCl2 Sigma M8266 Fina Biosolutions LLC AMINO-DEXTRAN 2000 KDA X150, 100MG Fisher Scientific NC1275021 Alexa Fluor 680 carboxylic acid, succinimidyl ester Thermofisher A20008 4% PFA Thermo Fisher J19943.K2 Sucrose Milipore-sigma S7903-1KG OCT compound Fisher Healthcare 23-730-571 Sodium azide Sigma S2002 Experimental models: Organisms/strains CX3CR1-GFP/+ mice Jax ID: 005582 B6.129P2(Cg)-Cx3cr1tm1Litt/J Software and algorithms ImageJ, Fiji Version 2.1.0/1.53c https://imagej.net/ij/ N/A ImageJ Plugin for Polymerase Chain Reaction:Article Title: Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling. Article Snippet: Article Basal spot junctions of Drosophila epithelial tissues respond tomorphogenetic forces and regulate Hippo signaling Plasmid Preparation:Article Title: Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling. Article Snippet: Article Basal spot junctions of Drosophila epithelial tissues respond tomorphogenetic forces and regulate Hippo signaling |
